Expression of miR-19a in HUVECs either transduced with Ad-miR19a and transfected with miR-19a inhibitor. (A) and (B), Quantitative real time PCR revealed that the expression levels of miR-19a were increased after Ad-miR-19a transduction (A), but suppressed by miR-19a inhibitor in a dose-dependent manner (B). Significance is indicated as *P < 0.05. (C) and (D), MiR-19a regulates ASK1 expression. HUVECs were transduced with Ad-miR-19a or Ad-LacZ (50MOI) for 24 h, and then treated with 100 ng/mL LPS for another 24 h. The expression of ASK1 was determined by quantitative real time PCR (C) and western blot (D).