Table 2 Comparison of reverse transcription (RT) and pre-amplification (PRE) variability
From: Pre-amplification in the context of high-throughput qPCR gene expression experiment
RND1 | CD83 | EIF3M | STK10 | FKBP | AVG var. | |
|---|---|---|---|---|---|---|
Efficiency (E) | 91% | 100% | 97% | 94% | 100% | |
Cq RT | 31.7 | 25.6 | 24.6 | 22.1 | 20.2 | |
\( S{D}_{\mathrm{RT}}=\sqrt{SD{(rt)}^2+SD{(qPCR)}^2} \) | 0.38 | 0.41 | 0.24 | 0.40 | 0.29 | |
Variability RT = (1 + E)SD RT | 28% | 33% | 18% | 17% | 22% | 23.6% |
Cq PRE* | 23.6 | 17.1 | 16.2 | 13.4 | 12.1 | |
\( S{D}_{PRE}=\sqrt{SD{(pre)}^2+SD{(qPCR)}^2} \) | 0.24 | 0.18 | 0.18 | 0.14 | 0.16 | |
Variability PRE = (1 + E)SD PRE | 17% | 13% | 13% | 10% | 12% | 13% |
Expression Differential | 1.2 | 0.7 | 1.0 | 0.7 | 1.3 |