Figure 1

Physiological and biochemical characterization. (A) Growth comparison on solid medium: Cells of the indicated strains were spotted onto LB agar in a serial dilution and incubated at the given temperatures. (B) Cells as indicated were grown at 20, 37 and 42°C to stationary phase. Growth rates were calculated and normalized values are given for each strain at each incubation temperature. Data were obtained from three independent experiments. Ribosomes from the indicated strains were isolated by sucrose cushion centrifugation and subjected to SDS-PAGE (C) and western blot analysis (D). For immunodetection, S15 and L1 specific antisera were used. Note that S15 wild type protein was not resolved by SDS-PAGE and immunoblotting. Asterisks denote unspecific protein bands.