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Figure 5 | BMC Molecular Biology

Figure 5

From: Efficient isolation of specific genomic regions retaining molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins

Figure 5

Detection of RNA associated with the c Pax5 locus. (A) Scheme of iChIP. After isolation of the cPax5 locus by iChIP using r3xFNLDD-D, the nascent RNA transcribed on the exon 1A of the cPax5 gene was detected by RT-PCR. (B) Results of iChIP using 0.5 μg of each r3xFNLDD-D and anti-FLAG Ab. % of input is shown. The error bar represents the range of duplicate experiments. (C) Detection of RNA corresponding to the exon 1A of the cPax5 gene but not that corresponding to the exon 3 of the cAID gene by RT-PCR.

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BMC Molecular Biology

ISSN: 1471-2199

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