Figure 5
Effects of down-regulating miR-100 on AKT/ERK/TrkB pathways in RGC-5 cells. RGC-5 cells were pre-treated with miR100-inhibitor, or miR-NC for 24 hours, followed by H2O2 (400 μM) treatment for another 24 hours. (A) Western blotting analysis was performed to examine the protein expressions of Thy-1, ERK1/2, phspho-ERK1/2 (pERK1/2), AKT, phospho-AKT (pAKT), TrkB and phosphorylated-TrkB (pTrkB), in miR100-inhibitor pre-treated and miR-NC pre-treated RGC-5 cells. Semi-quantitative examination was performed to compare the phosphorylation levels of TrkB (B), AKT (C) and ERK (D). (*, P <0.05)