Methylation of the LRRC4 promoter in glioma. A. Methylation of the LRRC4 promoter in glioma cell lines SF126 and SF767. DNA from the indicated glioma cell lines was bisulfite-modified and analyzed for methylation of the LRRC4 promoter by methylation-specific PCR analysis. The presence of a PCR product in lane U indicates unmethylated LRRC4. The presence of a PCR product in lane M indicates the methylated LRRC4. B. Schematic presentation of methylation analysis of the LRRC4 gene promoter using MSP in tumor samples (T) taken from patients with glioma and three normal brain tissue samples (N).