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Figure 4 | BMC Molecular Biology

Figure 4

From: Characterization of the internal IRES element of the zebrafish connexin55.5 reveals functional implication of the polypyrimidine tract binding protein

Figure 4

Polypyrimidine tracts and polypyrimidine tract binding protein (PTB) regulate the expression of p11-CT. A) Western blot of wild type Cx55.5-EGFP (WT) and PPT1 and PPT2 deleted (WT-PPTDel3) fusion constructs transiently transfected in N2A cells in the presence of either endogenous PTB (-) or overexpressed PTB (+). Immunodetection was performed by using anti-GFP antibody as described above. B) Western blot detection of endogenous and huPTB using anti-PTB antibody as described above and with β-actin as loading control.

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BMC Molecular Biology

ISSN: 1471-2199

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