Table 1 List of intron-spanning primers used in real-time RT-PCR
target mRNA | primer name | oligonucleotides sequence (5' → 3' orientation) |
|---|---|---|
mono ADP-ribosyltransferase 1 (ART1) | ART1-rt_fwd ART1-rt_rev | CAG GGG CTA CTC CTT TTT CC CCC AGA CCT GCA CTT CTT TT |
mono ADP-ribosyltransferase 3 (ART3) | ART3-rt_fwd ART3-rt_rev | AAT GGT CAC CAC GCT GCT CCC TCT TCA TCT GCG GAA TA |
mono ADP-ribosyltransferase 5 (ART5) | ART5-rt_fwd ART5-rt_rev | TGT GTC CTC AAG AGC AGT CG CA ACT CTG GTT GGA CAG GT |
hypoxanthine phosphoribosyltransferase 1 | Hprt1_fwd Hprt1_rev | CCA ACT TTG CTT TCC CTG GT CTG GCC TGT ATC CAA CAC TTC |
beta-2-microglobulin | β2m_fwd β2m_frev | CTG ACC GGC CTG TAT GCT AT TTT TCC CGT TCT TCA GCA TT |