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Table 2 PCR primers for quantitative chromatin immunoprecipitation assays

From: FIZ1 is part of the regulatory protein complex on active photoreceptor-specific gene promoters in vivo

Gene

ChIP Antibody

Positions†

Sense primer

Anti sense primer

bp

Rho

Pol-II-S2

+3051 to +3199

ggagatcccatgcacaaagt

taagctccctgccacttgac

149

Rho

FIZ1

-711 to -570

cagcaggccagtaggatca

gaggaggggcgtaagaagtt

141

Rho

FIZ1

-12 to +130

cccctctgcaagccaatta

gcaactccaggcactgac

142

Untr6

Pol-II-S2 FIZ1

4.4 × 106 bp downstream of Rho

tcaggcatgaaccaccatac

aacatccacacgtccagtga

216

  1. †Relative to the transcriptional start site (+1).
  2. Rho (Rhodopsin), Untr6 (untranslated region Chromosome-6), Pol-II-S2