Figure 9

Schematic of the REN colony screen. (A) The donor plasmid pBKOα is co-transfected with one of the piggyBac expression plasmids (fig. 8), transformed into E. coli and plated. (B) Precise excision of the piggyBac cassette removes the lacZ open reading frame. Digestion with Bgl II removes any excess piggyBac expression plasmids and donor plasmids which have not undergone excision. Excision events are scored as white colonies. Plating a portion of undigested plasmids and scoring blue colonies yields the total number of potential donor plasmids.