Figure 10

Schematic of the blue/white colony excision assay. (A) The donor plasmid pCR2.1-piggyBac{SV40} is co-transfected with one of the piggyBac expression plasmids (fig. 8) transformed into E. coli and plated. (B) Precise excision of the piggyBac{SV40} cassette restores the original lacZ open reading frame at the original 'TTAA' insertion site. By plating on kanamycin media containing IPTG and X-gal, only E. coli containing donor plasmids will develop into colonies. Colonies which are blue represent excision events, while the number of blue and white colonies represents the total number of potential donor plasmids.