Figure 7

Placement of the three primer pairs, relative to the target gene. The designed vectors allow for the reuse of primer pairs for multiple constructs, eg. targeted replacement and in locus overexpression. (A) Placement of the three primer pairs. (B) Construction of the vector for targeted gene replacement and homologous recombination with the genomic target. (C) Construction of the vector for in locus overexpression and homologous recombination with the genomic target, resulting in insertion of the gpdA promoter in front of the coding sequence for the target gene.