PSMB2 and RPL32 are suitable denominators to normalize gene expression profiles in bronchoalveolar cells

BMC Molecular Biology

Table 3 Descriptive and correlation analysis for ten housekeeping genes in the 1st cohort obtained by BestKeeper statistical applet.

	RPL32*	GAPDH	ACTB	GPS1	ARF1
GM [CTt]	18.65	20.44	17.92	24.86	22.15
AM [CTt]	18.69	20.53	17.99	24.91	22.20
Min [CTt]	15.90	13.10	14.30	22.20	18.80
Max [CTt]	22.00	24.60	21.60	28.50	25.60
SD [± CTt]	0.92	1.34	1.21	1.24	1.14
CV [% CTt]	4.93	6.51	6.73	4.99	5.12
Min [x-fold]	-6.73	-161.54	-12.31	-6.34	-10.21
Max [x-fold]	10.19	17.93	12.80	12.43	10.92
SD [± x-fold]	1.89	2.53	2.31	2.37	2.20
	PSMD2	G6PD	GNB2L1	CANX	PSMB2*
GM [CTt]	25.55	24.36	21.97	24.42	18.65
AM [CTt]	25.63	24.42	22.05	24.48	18.69
Min [CTt]	21.40	20.90	17.60	20.50	15.90
Max [CTt]	29.20	28.40	25.50	28.30	22.00
SD [± CTt]	1.67	1.34	1.54	1.25	0.92
CV [% CTt]	6.51	5.48	7.00	5.10	4.93
Min [x-fold]	-17.81	-11.03	-20.63	-15.17	-6.73
Max [x-fold]	12.51	16.42	11.58	14.69	10.19
SD [± x-fold]	3.18	2.53	2.91	2.38	1.89

Definition of abbreviations: GM [CTt], geometric mean of CTt; AM [CTt], arithmetic mean of CTt; Min [CTt] and Max [CTt], minimum and maximum values of CTt; SD [± CTt], standard deviation of the CTt; CV [%CTt], coefficient of variance expressed as a percentage on the CTt level. Min [x-fold] and Max [x-fold]: the extreme values of expression levels expressed as an absolute x-fold over- or under- regulation coefficient; SD [± x-fold]: standard deviation of the absolute regulation coefficients.
* Stably expressed genes were selected according to the criteria (SD [± CTt] < 1) published in Pfaffl et al [25].

ISSN: 1471-2199