Figure 4

Panel A: Computer search of vitamin D response elements (VDREs) in the rat insulin receptor (IR) gene promoter. Potential VDRE sequences in the rat IR gene promoter were identified by their homology with two consensus VDRE sequences using the SEQFIND programme developed in our laboratory. Nucleotides identical to the first VDRE consensus are labelled by filled circles (●) and nucleotides identical to the second VDRE consensus are labelled by empty circles (○). Panel B: Computer search of AP-2-like sites in the rat insulin receptor (IR) gene promoter. Potential AP-2-like sites in the rat IR gene promoter were identified by their homology with the AP-2 consensus (5'GCCN3GGG3') [32] using the SEQFIND programme. We detected 17 AP-2-like sites showing a difference of only 1 base from this consensus. Of these, only 7 AP-2-like sites flanking or overlapping the potential VDRE sequences in panel A are shown. Nucleotides identical to the AP-2 consensus are labelled by stars (⋆). Panel C: Candidate VDRE sequences overlapped by AP-2-like sites in the rat insulin receptor gene promoter. Nucleotides identical to the first VDRE consensus are labelled by filled circles (●) and nucleotides identical to the second VDRE consensus are labelled by empty circles (○)