Figure 6

Endogenous BCL2L11(BIM) transcripts comprising E1, E2 and 3'coding sequences are coordinately regulated by TSA in HEK293 cells. For simplicity the alias BIM is used in the figure instead of the approved gene symbol BCL2L11. A. Time course of expression of Exon 1-containing BIM transcripts in HEK293 cells following TSA treatment as measured by real-time PCR, using an E1-specific primer set (E1); TSA rapidly induces BIM-E1 expression at 4 hrs at both concentrations tested. B. The same analysis was performed using primer sets specific for the (previously known) BIM Exon 2; The expression of the P2-derived BIM transcript in HEK293 cells (E2) is induced by TSA at 4 hrs at the concentrations of 0.2 μM (i) and 1 μM (ii), as for E1 containing BIM transcripts. C. Time course of expression of BIM transcript levels in HEK293 cells treated with 0.2 μM TSA, and detected using primers specific for a 3' region of the coding sequence (and presumably recognizing most BIM transcripts; Total) or primers specific for Exon 1- or Exon 2-bearing BIM transcripts (E1 or E2, respectively).