Mac-1 is upregulated after ACA treatment. Scatterplots showing Mac-1 mRNA levels in the different treatment groups. Compared to the sham treated group the expression of the microglia marker Mac-1 is 9.3 ± 2.34 -fold upregulated four days after the insult in the asphyxial cardiac arrest group (p-value 0.006) and 14.9 ± 4.56 -fold in the ACA with minocycline treatment group (p-value 0.001) (A). Seven days after treatment Mac-1 is 10.3 ± 6.18 -fold (p-value 0.011) and 9.2 ± 4.84 -fold (p-value 0.001) upregulated in the ACA and in the ACA with minocycline treatment groups, respectively (B). The increased Mac-1 expression is detectable 21 days after the insult too (14.3 ± 9.17-fold, p-value 0.024 and 22.2 ± 9.93-fold, p-value 0.006) in the ACA and ACA with minocycline treatment groups, respectively (C). Calculations were done using REST-MCS software.