B2m is unsuitable as an endogenous control gene after ACA treatment. Scatterplots showing B2m mRNA levels in the different treatment groups.B2m expression in hippocampus is upregulated due to asphyxial cardiac arrest 4 days (A) and 21 days (C) after treatment. Though not significant, there is a tendency for B2m upregulation 7 days after ACA too (B). Compared to the sham treated group B2m expression is 1.7 ± 0.30 -fold upregulated in the ACA group (p-value 0.038) and 2.0 ± 0.39 -fold upregulated in the ACA with minocycline treatment group (p-value 0.009), respectively four days after the injury. Twenty-one days after treatment B2m is 2.4 ± 0.49 -fold upregulated in the ACA with minocycline treatment group (p-value 0.020) vs. sham operated group. Absolute gene regulation value, standard error and p-value are given as calculated using REST-MCS software.