Figure 3

A part of the carboxy-terminal domain of Cx55.5 is translated from an internal translation site within the coding region of Cx55.5: Schematic view of wild type full length WT (I), frameshift mutated (position 1179) full length, p11-CT (II), and in-frame ATG replaced by GCG (position 1201) of full length Cx55.5 construct, FL (III). The frameshift mutation (II) leads to a premature stop codon at nucleotide position 1227. The carboxy-terminus of Cx55.5 is truncated by 90 amino acids and 15 amino acids are altered after the added "T" at nucleotide position 1179. B) Western blot of transiently transfected N2A cells: (lane I) Wild type (WT), (lane II) frameshift mutated p11-CT, and (lane III) ATG replaced by GCG of full length Cx55.5 (FL).