R. BsaHI Sequence Alignment. MAFFT alignment of R. BsaHI and its homologues. Residues are coloured where the aligned sequences share at least 60% identity. Proposed conserved and catalytic motifs from the PD-(D/E)xK superfamily are indicated. Also shown is the JNet secondary structure prediction, where green arrows indicate β-sheets and red tubes are predicted α-helices. The JNet prediction confidence parameter, JNETCONF, is shown below the structure prediction. Residues targeted for mutational analysis are indicated with *. Figure generated using Jalview .