Table 2 List of primers and PCR conditions used for making luciferase constructs

Fal1Luc 1 kb F

CTCGAGATTCATTCTGTGTCATCTGT

94°C 1'

53°C 1.30'

68°C 1.30'

Fal1Luc 0.5 kb F

CTCGAGGCCTTTTCTTTCTATTTTAAGC

94°C 1'

53°C

68°C 1'

Fal1Luc R

CCATGTTGTAATCCATGGATTTTTTAT

Fal2Luc 2 kb F

CTCGAGTATATTGTATGTAATTTGAGATAATGA

94°C 1'

52°C 1.30'

62°C 2'

Fal2Luc 1 kb F

CTCGAGTGAATATATAGAAACCTCACTAGG

94°C 1'

55°C 1.30'

68°C 1.30'

Fal2Luc 0.5 kb F

CTCGAGAATTCATTGCATTTATGTGTTTTCTA

94°C 1'

53°C 1'

68°C 1'

Fal2Luc R

CATGTGGTAATCCATGGTCTTCAAC

Fal2aLuc 2 kb F

CTCGAGGATGATTTCGCCTTTTATAG

94°C 1'

50°C 2'

65°C 2'

Fal2aLuc 1 kb F

CTCGAGTATGAATATACATATACACTAGG

94°C 1'

52°C 1.30'

65°C 1.30'

Fal2aLuc 0.5 kb F

CTCGAGAAAGATAAGCAACATCGATT

94°C 1'

53°C 1'

68°C 1'

Fal2aLuc R

CATGTGGTAATCCATGGTTAAAA

Fal3Luc 2 kb F

CTCGAGCATACAGTTGAAGGGATGATG

94°C 1'

52°C 1.30'

62°C 2'

Fal3Luc 1 kb F

CTCGAGACAAAGAAGAATATGAACAAAG

94°C 1'

55°C 1.30'

68°C 1.30'

Fal3Luc 05.kb F

CTCGAGTTCGAAAATTTCCGTATACAT

94°C 1'

53°C 1'

68°C 1'

Fal3Luc R

CATATGATATTCCATGGTTCAAAC