Characterization of two functional NKX3.1 binding sites upstream of the PCAN1 gene that are involved in the positive regulation of PCAN1 gene transcription

Table 1 PCR primers used in chromatin immunoprecipitation

Names	Sequences	Product sizes
NBS1 primers	F: GATTCTTTGACTGGTCTGGCACAC
	R: TTATCCATTGTGCCTGGAGCTGAG	170 bp (spanning NBS1)
BNS2 primers	F: TCCTACTAACGGCATGTAAGGAGG
	R: ATTGCCATGTCTGGACTGTGAGTG	170 bp (spanning NBS2)
NBS3 primers	F: AAGAATGAGCTGATCCTCCTATGC
	R: GGTTTAGTAATAGACTGGGCACCA	150 bp (spanning NBS3)
NBS4,5 primers	F: GTGTAGCAGGTAAATCAGTGTGAG
	R: TCAGCTGACGAGCAACTTCAATTC	180 bp (spanning NBS4 and 5)

NBS1 F and R span NBS1;
NBS2 F and R span NBS2; NBS3 F and R span NBS3; BNS4,5 F and R span NBS4 and NBS5

ISSN: 1471-2199