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Figure 18 | BMC Molecular Biology

Figure 18

From: Molecular characterization of senescence marker protein-30 gene promoter: Identification of repressor elements and functional nuclear factor binding sites

Figure 18

Electrophoretic mobility shift assay to confirm the binding of C/EBP transcription factor. Lane 1–5, labeled mutated C/EBP oligonucleotide duplex with 6 μg RLNE; Lane 2, C/EBPβ antibody; Lane 3–5, describe the competition with 100 fold molar excess of unlabeled homologous self, SMP-C/EBP and C/EBP consensus. Lane 6–11, labeled SMP-C/EBP oligonucleotide duplex with 6 μg RLNE; Lane 7, C/EBPβ antibody; Lane 8–11, describe the competition with 100 fold molar excess of unlabeled homologous self, C/EBP consensus, Mut SMP-C/EBP and nonspecific SRY oligonucleotide duplex respectively. Antibody shift is seen with SMP-C/EBP only and not with mutated SMP-C/EBP.

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BMC Molecular Biology

ISSN: 1471-2199

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