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Figure 16 | BMC Molecular Biology

Figure 16

From: Molecular characterization of senescence marker protein-30 gene promoter: Identification of repressor elements and functional nuclear factor binding sites

Figure 16

Reporter activity of site direct mutated Sp1, site direct mutated C/EBP and site direct mutated Sp1 and C/EBP sites reporter constructs along with wild type Luc 5 on right site and the schematic representation of the mutated Sp1 and C/EBP sites along with wild type Luc 5 sites on left site. All transfections were repeated in duplicates and the results are expressed as the mean of three different experiments ± S.D Approximate location of the transcription factor binding to wild type Luc 5 and site directed mutated construct are shown. A significant decrease (~16%) in reporter activity of mutated Sp1 construct is seen as compared with wild type Luc 5. There is no significant change in reporter activity with mutated C/EBP construct. Double mutation of Sp1 and C/EBP enhance the reporter activity by 23% as compared to wild type Luc 5.

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BMC Molecular Biology

ISSN: 1471-2199

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