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Figure 12 | BMC Molecular Biology

Figure 12

From: Molecular characterization of senescence marker protein-30 gene promoter: Identification of repressor elements and functional nuclear factor binding sites

Figure 12

DNase I footprinting pattern of the repressor region (-513 to -352). Lane1,2 and 9,10 refer to sequencing reaction ladder obtained by taking the same labeled primer used in PCR for DNase I footprinting. Lane 3,4,5 represent DNA treated with DNase I in absence of nuclear protein. Lane 6,7,8 represent DNA treated with DNase I in presence of 50 μg rat liver nuclear extract (RLNE). The DNase I protected sites are marked on right site and the sequences are given in table 3. DNase I footprinting was carried out with fragments generated by labeled forward primer.

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BMC Molecular Biology

ISSN: 1471-2199

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