DNA demethylation-dependent enhancement of toll-like receptor-2 gene expression in cystic fibrosis epithelial cells involves SP1-activated transcription

Table 2 Methylation of CpG sites in the TLR2 promoter is significantly different between non-CF and CF epithelial cells^a

CpG#		Sequences	Methylated	Unmethylated	p values
#1–3	non-CF	43	46	83
	CF	41	17	106	7.277 × 10^-5***
#4–5	non-CF	43	18	68
	CF	41	22	60	0.4698
#6–8	non-CF	43	8	121
	CF	41	1	122	0.0362*
#9–10	non-CF	43	3	83
	CF	41	1	81	0.6209
#11–12	non-CF	43	6	80
	CF	41	1	81	0.1179
#13–15	non-CF	43	20	109
	CF	41	8	115	0.0273*
#16–17	non-CF	43	12	74
	CF	41	3	79	0.0282*
#18–20	non-CF	43	22	107
	CF	41	4	119	0.0003***

^aCpG methylation was scored at specific sites (CpG#1-3, 4–5, 6–8, 9–10, 1–12, 13–15, 16–17 and 18–20) in the TLR2 promoter sequence from non-CF (16HBE14o-, n = 23; HeLa, n = 10; A549, n = 10) and CF (CFBE41o-, n = 21; CFTE41o-, n = 10; CFPAC-1, n = 10) epithelial cells. The number of methylated and unmethylated sites was scored and a Fischer's exact test was used to assign p values (*, p < 0.05; ***, p < 0.001).

ISSN: 1471-2199