Table 1 Oligonucleotides used for this study
Primers used for the generation of TLR2 promoter/reporter constructs | ||
|---|---|---|
pGL3-T2P(-309) | 5'-GCCTCTAGCGTCTCGATTCGC-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
pGL3-T2P(-194) | 5'-CTGTCGCAGCCTAGCTCACGG-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
pGL3-T2P(-120) | 5'-CCTGCCTGGAACTCAGCGCG-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
pGL3-T2P(-60) | 5'-TGCCCCGTGGAAGGGGCGGTT-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
pGL3-T2P(-12) | 5'-CGCGCGGACTTTCCCTTTTGC-3' | 5'-CTGGGAGAACTCCGAGCAGTC-3' |
Primers used for the generation of mutant TLR2 promoter/reporter constructsa | ||
pGL3-T2P(-120) SPm | 5'-CCCGTGGAAGGTTA GGTTCCCGCACCCCAG-3' | 5'-CTGGGGTGCGGGAACCTAA CCTTCCACGGG-3' |
pGL3-T2P(-120)/(-60) ETSm | 5'-GCGCACGTGCCCCGAC GAAGGGGCGGTTCC-3' | 5'-GGAACCGCCCCTTCGT CGGGGCACGTGCGC-3' |
Primers used for semi-quantitative RT-PCR | ||
SP1 | 5'-TGTGAATGCTGCTCAACTCTCC-3' | 5'- CATGTATTCCATCACCACCAG -3' |
SP3 | 5'-AACCTGATCCTGAAGAGTGGC-3' | 5'-TGGCGGAAGTATTAACAGTTCC-3' |
Oligonucleotides used for EMSAb | ||
T2P(-64/-31) | 5'-GTGCCCCGTGGAAGGGGCGG TTCCCGCACCCCAG-3' | 5'-CTGGGGTGCGGGAACCGCCCC TTCCACGGGGCAC-3' |
T2P(-64/-31) SPm | 5'-GTGCCCCGTGGAAGG TTA GG TTCCCGCACCCCAG-3' | 5'-CTGGGGTGCGGGAACC TAA CC TTCCACGGGGCAC-3' |
T2P(-64/-31)-monoMe | 5'-GTGCCCCGTGGAAGGGGCGG TTCCCGCACCCCAG-3' | 5'-CTGGGGTGCGGGAAC/M-C/GCCCC TTCCACGGGGCAC-3' |
T2P(-64/-31)-triMe | 5'-GTGCCCCGTGGAAGGGGCGG TTCCCGCACCCCAG-3' | 5'-CTGGGGTG/M-C/GGGAAC/M-C/GCCCC TTCCA/M-C/GGGGCAC-3' |