Figure 3

Uri is cytoplasmic when expressed in COS7 cells, and can cause re-localisation of PP1α. FLAG-tagged Uri (A), URI/RMP (B) or URI/RMP-D2 (C) were expressed in COS7 cells and visualised by immunofluorescence. Uri and URI/RMP were predominantly cytoplasmic, with a perinuclear concentration and weak nuclear staining. URI/RMP-D2 truncated protein predominantly localised to the nucleus, although cytoplamic labelling was also seen. D-F, COS7 cells transfected with FLAG-Uri (red) and HA-PP1α87B (green). The cell indicated by an arrow is only expressing HA-PP1α87B, and shows that HA-PP1α87B is nuclear when expressed in COS7 cells. The cell indicated by the arrowhead is expressing both HA-PP1α87B and FLAG-Uri, and shows a predominantly cytoplasmic co-localisation for Uri and HA-PP1α87B.