Table 1 Oligonucleotides used in this study. The assay-specific annealing temperatures are given in the bottom right column. Differing bases among members of the let-7 family are indicated in red. Binding sequences for universal primers are underlined and the miRNA specific sequences are indicated in bold.

let-7a

uga ggu agu agg uug uau agu u

let-7b

uga ggu agu agg uug ugu ggu u

let-7c

uga ggu agu agg uug uau ggu u

miR-16

uag cag cac gua aau auu ggc g

miR-21

uag cuu auc aga cug aug uug a

miR-23b

auc aca uug cca ggg auu acc

miR-27a

uuc aca gug gcu aag uuc cgc

miR-141

uaa cac ugu cug gua aag aug g

miR-145

guc cag uuu ucc cag gaa ucc cuu

miR-200c

uaa uac ugc cgg gua aug aug g

RT6-let-7a

tgt cag gca acc gta ttc acc gtg agt ggt aac tat

RT6-let-7b

tgt cag gca acc gta ttc acc gtg agt ggt aac cac

RT6-let-7c

tgt cag gca acc gta ttc acc gtg agt ggt aac cat

RT6- miR-16

tgt cag gca acc gta ttc acc gtg agt ggt cgc caa

RT6- miR-21

tgt cag gca acc gta ttc acc gtg agt ggt tca aca

RT6- miR-23b

tgt cag gca acc gta ttc acc gtg agt gct ggt aat

RT6- miR-27a

tgt cag gca acc gta ttc acc gtg agt ggt gcg gaa

RT6- miR-141

tgt cag gca acc gta ttc acc gtg agt ggt cca tct

RT6- miR-145

tgt cag gca acc gta ttc acc gtg agt ggt aag gga

RT6- miR-200c

tgt cag gca acc gta ttc acc gtg agt ggt cca tca

short-let-7a-rev

cgt cag atg tcc gag tag agg ggg aac ggc gtg agg tag tag gtt gta ta

short-let-7b-rev

cgt cag atg tcc gag tag agg ggg aac ggc gtg agg tag tag gtt gtg tg

short-let-7c-rev

cgt cag atg tcc gag tag agg ggg aac ggc gtg agg tag tag gtt gta tg

short-miR-16-rev

cgt cag atg tcc gag tag agg ggg aac ggc gta gca gca cgt aaa ta

short-miR-21-rev

cgt cag atg tcc gag tag agg ggg aac ggc gta gct tat cag act ga

short-miR-23b-rev

cgt cag atg tcc gag tag agg ggg aac ggc gat cac att gcc agg g

short-miR-27a-rev

cgt cag atg tcc gag tag agg ggg aac ggc gtt cac agt ggc taa g

short-miR-141-rev

cgt cag atg tcc gag tag agg ggg aac ggc gta aca ctg tct ggt aaa g

short-miR-145-rev

cgt cag atg tcc gag tag agg ggg aac ggc ggt cca gtt ttc cca gga a

short-miR-200c-rev

cgt cag atg tcc gag tag agg ggg aac ggc gta ata ctg ccg ggt aa

5S rRNA fw

gcc cga tct cgt ctg atc t

5S rRNA rev

agc cta cag cac ccg gta tt

MP-fw

tgt cag gca acc gta ttc acc

MP-rev

cgt cag atg tcc gag tag agg