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Figure 2 | BMC Molecular Biology

Figure 2

From: miR-Q: a novel quantitative RT-PCR approach for the expression profiling of small RNA molecules such as miRNAs in a complex sample

Figure 2

Amplification plot and melting curve of the proposed miR-145 miR-Q assay. A) Amplification plot of synthetic miR-145 with 50 ng bacterial total RNA present as complex background. Target input ranged between 2 nM and 0.2 fM in a miR-Q reaction. B) SYBR green melting curve of the miR-145 assay. Neither the non-spiked bacterial total RNA control, nor H2O control showed a background signal over 40 cycles.

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BMC Molecular Biology

ISSN: 1471-2199

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