Figure 6
Structure prediction and primer extension on mRNAs synthesized by different plasmids. The structure of the transcripts starting at the sigA promoter in pMYS739, pMYS740, pMYS743 and pMYS749 were obtained with the m-fold structure prediction program of Zuker [41] and primer extension analyses were performed on RNAs extracted from mc2155 transformed with the different plasmids, with oligonucleotide 809. Sequence reactions obtained with the same oligonucleotide were run in the same gel. The position of the sigA promoter, the 443 processing site and the PPS region are indicated on the right. The arrows indicate the position of the cut on the sequence structure. The mutations are circled.