Table 1 Primer and probe sequences

Pitx2a/b.forward

5' CGTACGAAGCTTATGGAGACCAATTGTCGCAAAC 3'

Pitx2c.forward

5' CGGGATCCTCCATGAACTGCATGAAAGGC 3'

Pitx2.reverse

5' AGTTGCCCACTCCGACAGTCCT 3'

Mutagenesis (sense strand)

Pitx2c.M35L

5' CATCCCCAGGCGTTAGCCTTAGCTTCGGTCCTAGCTCCTG 3'

Pitx2c.L5stop

5' CTCCATGAACTGCATGTAAGGCCCGCTGCCCTTG 3'

Pitx2c.T-3A

5' CGATTCGGGATCCACCATGAACTGCATG 3'

Oligonucleotide probes for colony hybridization

P1 probe

5' CCAGCAGCAAGCTGTTCCCGCGGCAGCACCCC 3'

P2 probe

5' GCCGGCAGCCGTTGAATGTCTCTTCTCC 3'

RT-PCR

Pitx2a/b.forward

5' CGTACGAAGCTTATGGAGACCAATTGTCGCAAAC 3'

Pitx2.reverse

5' AGTTGCCCACTCCGACAGTCCT 3'

Pitx2c.forward

5' CGGGATCCTCCATGAACTGCATGAAAGGC 3'

Pitx2.HD.reverse

5' TCGGGCTTCCGTAAGGTTGGTC 3'

Rpl19.forward

5' CTGAAGGTCAAAGGGAATGTG 3

Rpl19.reverse

3' GGACAGAGTCTTGATGATCTC 3'

EMSA (sense strand)

-61/-40 mFshb

5' CACCCAGTAAATCC ACAGGGTT 3'

CE3 rPomc

5' ACCAGGATGCTAAGCC TCTCGTC 3'

-106/-76 bLhb

5' GCCCCCGGGGAGATTA GTGTCCAGGTTACCCCACC 3'

-321/-280 mGnrhr

5' TCATTAAGGCTAATTGGATGATATTATGAGTCACTTTCGACA 3'

-406/-365 mGnrhr

5' TTTTAAATTGGATCGGGATTTTTAAATTACTTTTCTGTATTT 3'

-100/-68 bCga

5' GGGTGGAATTTACTGTTGATCCCTGGACTTAGA 3'