Figure 5

Upregulation of DYRK1A mRNA by E2F1 overexpression. A) Phoenix cells were transfected with an expression plasmid for E2F1 (2 μg/10 cm plate) or vector DNA (Ctrl). Total RNA was isolated 2 days after transfection and subjected to Northern blot analysis with probes for DYRK1A and GAPDH as indicated. Ethidium bromide-stained bands of the ribosomal RNAs are shown as a loading control. B) Saos2 cells expressing E2F1 under the control of a doxycycline-inducible promoter were treated for 18 h with doxycycline (E2F1), or were not treated (Ctrl) before the RNA was isolated. Duplicate lanes contain RNA from parallel plates.