Table 1 The ranking of candidate reference genes according to their expression stability by geNorm and NormFinder in Senegalese sole, Atlantic halibut and combined analysis.
Rank | GeNorm | Rank | NormFinder | ||||
|---|---|---|---|---|---|---|---|
S. senegalensis | H. hippoglossus | Combined | S. senegalensis | H. hippoglossus | Combined | ||
1/2 | UBQ-eEF1A1 | RPS4-RPL13a | UBQ-eEF1A1 | 1 | GAPDH2 | RPS4 | RPS4 |
3 | GAPDH2 | eEF1A1 | RPS4 | 2 | RPS4 | UBQ | UBQ |
4 | RPS4 | UBQ | HPRT1 | 3 | ACTB2 | RPL13a | eEFA1 |
5 | ACTB2 | ACTB2 | ACTB1 | 4 | eEF1A1 | GST | GST |
6 | ANXA2 | HPRT1 | GST | 5 | UBQ | HPRT1 | HPRT1 |
7 | HPRT1 | GST | GAPDH1 | 6 | ANXA2 | eEF1A1 | ACTB1 |
8 | 18S rRNA | 18S rRNA | 18S rRNA | 7 | HPRT1 | ACTB2 | GAPDH1 |
9 | ACTB1 | ACTB1 | ANXA2 | 8 | GST | 18S rRNA | 18S rRNA |
10 | GAPDH1 | ODC | 9 | GAPDH1 | ACTB1 | ANXA2 | |
11 | GST | GAPDH1 | 10 | ACTB1 | ODC | ||
12 | RPL13a | ANXA2 | 11 | 18S rRNA | GAPDH1 | ||
12 | RPL13a | ANXA2 | |||||