Figure 5
Evaluation of the presence of the plasminogen system in hMADS cells. (A) PAI-1 mRNA levels have been determined in hMADS cells at day 0, day 3 adipocytes (adipo) and day 3 osteoblasts (osteo). (B) PAI-1 and (C) tPA protein levels have been measured by ELISA in the secretion media of hMADS cells at day 0, day 3 adipocytes (adipo) and day 3 osteoblasts (osteo) after 6 h of incubation. *: p < 0.05. (D) Zymographic analysis of plasminogen activators activity in hMADS cell conditioned media collected after 6 h of incubation. A representative casein-plasminogen zymogram out of three independent experiments is shown. (E) RT-PCR analysis of UPAR expression in hMADS cells at day 0, day 3 adipocytes and day 3 osteoblasts. β-actin expression is reported as internal control. PCR products have been separated on a 1% agarose gel.