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Figure 3 | BMC Molecular Biology

Figure 3

From: A novel mutant of the Sup35 protein of Saccharomyces cerevisiae defective in translation termination and in GTPase activity still supports cell viability

Figure 3

Mass spectrometry analysis of phosphorylated Sup35p. (a) On-target dephosphorylation. Detail of the total tryptic digest mass spectra prior and after alkaline phosphatase treatment. The 80 Da shift is the typical signature for a mono-phosphorylated peptide. (b) PSD-MALDI spectrum of the CAF-derivatised peptide [339–349] (HPLC fraction n°22). The neutral loss of H3PO4 (98 Da) converts pThr into dehydroamino-2-butyric acid produced by β-elimination and the "CAF" gap corresponds to the initial loss of 136 Da from the sulphonic acid group.

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BMC Molecular Biology

ISSN: 1471-2199

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