Relative SseEF1A expression levels as determined by real-time quantitative PCR in the untreated (grey) and TU-treated (black) groups. Untreated and TU-treated samples were collected for RNA isolation at four different times: 8 hours, and 6 days, 11 days, and 15 days after treatment starting on 7 DPH. Expression values were normalized to those of GAPDH2. Data are expressed as the mean fold change (mean ± SEM, n = 3) from the calibrator group (control 8 hours). Values marked with an asterisk are significantly different (P < 0.05 or less) from the corresponding untreated control group values.