Effects of Wnt3aCM and Wnt5aCM on Canonical Wnt signaling in L, A549, and H441 cells. Cultured L, A549, or H441 cells were transfected with pSV-β-gal (Promega, WI) and STF plus Ror2WT (+Ror2) or the control vector (-Ror2). Twenty four hours after transfection, cells were treated with L-CM, Wnt3aCM or Wnt5aCM. Wnt3aCM and Wnt5aCM were used at 1/8 and 1/4 dilutions, respectively. Equal amount of conditioned media was used in each assay. Luciferase values representing the STF activities were normalized to beta-galactosidase for transfection efficiency. Values of the assays treated with L-CM alone (#1 and #5) were adjusted to unity to normalize all other experimental values in the same group. CM: conditioned medium. * indicates p < 0.05.