Figure 8

Tc UBP1 and Tc RBP3 bind to a transcript containing alternative RNA motifs. A) Scheme showing the spatial distribution of additional motifs in 3' UTR of specific transcripts. 1 and 3, represent a region where different motifs exist for Tc UBP1 and Tc RBP3, respectively; 1+3, represent regions where overlapping motifs for both proteins were found. Lines below them represent a motif. B) Scheme of PARB1 mRNA and its 3' UTR containing additional motifs for each protein (named UBP1-m14 and RBP3-m2). The numbers indicate distances from stop codon where motifs were found. C) RT-PCR assays in IP samples shows that PARB1 is a common target. Biotin pull-down assay was used to asses the binding of both proteins to the following regions of PARB1 mRNA: a) with both motifs b) without motifs.