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Figure 5 | BMC Molecular Biology

Figure 5

From: Functionally related transcripts have common RNA motifs for specific RNA-binding proteins in trypanosomes

Figure 5

Deletions and mutations of motifs sequences hinder the interaction with proteins. Biotin pull-down assays using shorter and mutated regions of ZFP1 and RpS5 mRNAs. Secondary structures of these RNAs are shown. ZFP1m and RPS5m are RNAs comprising flanking regions plus UBP1m or RBP3m, respectively. ZFP1m(-) and RPS5m(-) contain only flanking regions and ZFP1m (mut) and RPS5m (mut) enclose irrelevant motifs. GST fusion proteins (rTc UBP1 and rTc RBP3) were recognized by Western blot.

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BMC Molecular Biology

ISSN: 1471-2199

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