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Table 2 Comparison of mean cycle threshold (Ct ± SD) values across different sample groups

From: Identification of Importin 8 (IPO8) as the most accurate reference gene for the clinicopathological analysis of lung specimens

 

Cultured cells

Clinical samples

 

Tumor

Normal

Tumor

Normal

18S

12.88 ± 0.61

11.24 ± 0.73

12.60 ± 1.62

13.79 ± 1.00

ACTB

22.20 ± 1.07

19.30 ± 0.26

23.07 ± 1.36

23.59 ± 1.15

B2M

24.99 ± 0.51

23.46 ± 0.43

23.21 ± 1.18

22.94 ± 0.75

GAPDH

22.61 ± 0.72

20.64 ± 0.00

24.48 ± 1.92

27.13 ± 1.11

GUSB

29.36 ± 0.73

28.61 ± 0.36

28.81 ± 1.36

29.77 ± 1.04

HMBS

29.23 ± 1.11

28.41 ± 0.45

30.89 ± 1.47

32.68 ± 0.76

HPRT1

27.07 ± 0.71

26.20 ± 0.40

29.10 ± 1.90

30,63 ± 0.97

IPO8

29.65 ± 1.19

28.97 ± 0.03

30.43 ± 1.37

30.91 ± 0.97

PGK1

24.97 ± 0.83

23.15 ± 0.32

25.23 ± 1.63

27.56 ± 0.92

POLR2A

27.95 ± 0.93

26.37 ± 0.06

28.55 ± 1.32

29.58 ± 1.06

PPIA

23.16 ± 0.82

21.49 ± 0.23

24.37 ± 1.47

26.00 ± 1.07

RPLP0

23.01 ± 0.71

21.02 ± 0.37

24.34 ± 1.82

26.01 ± 0.98

TBP

30.95 ± 1.75

29.00 ± 0.96

31.79 ± 2.12

32.59 ± 1.16

TFRC

26.71 ± 0.76

26.64 ± 0.13

27.94 ± 2.18

28.65 ± 1.06

UBC

23.11 ± 0.62

20.83 ± 0.10

23.82 ± 2.29

24.78 ± 1.48

YWHAZ

29.25 ± 0.68

26.72 ± 0.28

29.47 ± 2.05

31.06 ± 0.85