Figure 5

FGF-2 and GFG protein expression in rat tissues. Representative immunohistochemical staining of FGF-2 (panels a-d) and GFG (panels e-h) in rat liver (upper four panels) and kidney (lower four panels). FGF-2 immunoreactivity in liver was exclusively cytoplasmic (a, ×400; b, ×400 zoom). In contrast, GFG protein expression was detected in both cytoplasm and nuclei (e, ×400; f, ×400 zoom). In kidney, FGF2 staining is seen in glomerular endothelial and peritubular nuclei, but not in podocytes, mesangium or tubular nuclei. (c, ×400; d, ×400 zoom). In panel D, an FGF-positive endothelial cell nucleus is circled, and FGF-negative nuclei of a mesangial cell (arrowhead) and podocyte (arrow) are indicated for comparison. GFG immunostaining was detected in glomerular endothelial and peritubular capillary nuclei, and also in the nuclei and cytoplasm of podocytes and tubules (g, ×400; h, ×400 zoom).