BMC Molecular Biology

Table 4 Inhibition effect of the RT contents of five commercial RT systems measured by qRT-PCR.

From: Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR

RT systems	Slope^a	SD	Efficiency^{a, b}		R ²	SD	Average IP^a
Sensiscript
E/R² - all points							14.7 ± 6.1%
	-3.393	± 0.030	97.13	± 5.00%	0.9981	± 0.0016
E/R² - w/o 1st point
	-3.464	± 0.041	94.42	± 1.53%	0.9983	± 0.0017
E/R² - w/o 1st 2 points
	-3.519	± 0.089	92.45	± 3.12%	0.9968	± 0.0029
Omniscript
E/R² - all points							31.2 ± 23.5%
	-3.227	± 0.054	104.14	± 2.42%	0.9958	± 0.0035
E/R² - w/o 1st point
	-3.323	± 0.057	99.98	± 2.37%	0.9957	± 0.0038
E/R² - w/o 1st 2 points
	-3.473	± 0.208	94.50	± 8.10%	0.9969	± 0.0019
SuperScript II
E/R² - all points							59.7 ± 6.8%
	-2.862	± 0.109	123.79	± 6.95%	0.9907	± 0.0029
E/R² - w/o 1st point
	-3.004	± 0.122	115.50	± 6.93%	0.9920	± 0.0013
E/R² - w/o 1–2 points
	-3.303	± 0.121	100.99	± 5.26%	0.9971	± 0.0034
SuperScript III
E/R² - all points							79.3 ± 2.9%
	-2.400	± 0.095	161.44	± 10.16%	0.9830	± 0.0057
E/R² - w/o 1st point
	-2.583	± 0.083	144.07	± 6.84%	0.9898	± 0.0040
E/R² - w/o 1–2 points
	-2.882	± 0.069	122.43	± 4.34%	0.9980	± 0.0024
PowerScript
E/R²- all points							69.3 ± 2.1%
	-2.568	± 0.063	145.27	± 5.49%	0.9693	± 0.0113
E/R² - w/o 1st point
	-2.759	± 0.168	131.12	± 12.38%	0.9694	± 0.0038
E/R² - w/o 1–2 points
	-3.203	± 0.141	105.50	± 6.71%	0.9817	± 0.0167

^aResults report the means of triplicate assays for each RT system.
^bAmplification efficiency is calculated using the equation E% = (1 - 10^(-1/slope)) × 100, where 100% PCR efficiency corresponds to a slope (Δ Ct) of -3.3; E and coefficient of correlation (R²) are derived from the slope of a sample's calibration curve; SD, standard deviation.

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