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Table 4 Inhibition effect of the RT contents of five commercial RT systems measured by qRT-PCR.

From: Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR

RT systems Slopea SD Efficiencya, b   R 2 SD Average IPa
Sensiscript        
E/R2 - all points        14.7 ± 6.1%
  -3.393 ± 0.030 97.13 ± 5.00% 0.9981 ± 0.0016  
E/R2 - w/o 1st point        
  -3.464 ± 0.041 94.42 ± 1.53% 0.9983 ± 0.0017  
E/R2 - w/o 1st 2 points        
  -3.519 ± 0.089 92.45 ± 3.12% 0.9968 ± 0.0029  
Omniscript        
E/R2 - all points        31.2 ± 23.5%
  -3.227 ± 0.054 104.14 ± 2.42% 0.9958 ± 0.0035  
E/R2 - w/o 1st point        
  -3.323 ± 0.057 99.98 ± 2.37% 0.9957 ± 0.0038  
E/R2 - w/o 1st 2 points        
  -3.473 ± 0.208 94.50 ± 8.10% 0.9969 ± 0.0019  
SuperScript II        
E/R2 - all points        59.7 ± 6.8%
  -2.862 ± 0.109 123.79 ± 6.95% 0.9907 ± 0.0029  
E/R2 - w/o 1st point        
  -3.004 ± 0.122 115.50 ± 6.93% 0.9920 ± 0.0013  
E/R2 - w/o 1–2 points        
  -3.303 ± 0.121 100.99 ± 5.26% 0.9971 ± 0.0034  
SuperScript III        
E/R2 - all points        79.3 ± 2.9%
  -2.400 ± 0.095 161.44 ± 10.16% 0.9830 ± 0.0057  
E/R2 - w/o 1st point        
  -2.583 ± 0.083 144.07 ± 6.84% 0.9898 ± 0.0040  
E/R2 - w/o 1–2 points        
  -2.882 ± 0.069 122.43 ± 4.34% 0.9980 ± 0.0024  
PowerScript        
E/R2- all points        69.3 ± 2.1%
  -2.568 ± 0.063 145.27 ± 5.49% 0.9693 ± 0.0113  
E/R2 - w/o 1st point        
  -2.759 ± 0.168 131.12 ± 12.38% 0.9694 ± 0.0038  
E/R2 - w/o 1–2 points        
  -3.203 ± 0.141 105.50 ± 6.71% 0.9817 ± 0.0167  
  1. aResults report the means of triplicate assays for each RT system.
  2. bAmplification efficiency is calculated using the equation E% = (1 - 10(-1/slope)) × 100, where 100% PCR efficiency corresponds to a slope (Δ Ct) of -3.3; E and coefficient of correlation (R2) are derived from the slope of a sample's calibration curve; SD, standard deviation.
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BMC Molecular Biology

ISSN: 1471-2199

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