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Table 3 Detection yield, copy number and cycle threshold obtained in qPCR for the commercial RT systems SuperScript III and PowerScript

From: Detection limits of several commercial reverse transcriptase enzymes: impact on the low- and high-abundance transcript levels assessed by quantitative RT-PCR

Background RNA (ng) 0 10 25 50 100 1000 2000
RT systemsa Datab        
SuperScript III         
  DY (%) 3.75 6.30 2.90 2.49 4.07 20.37 12.37
Low copy copy num. ± SD 10 ± 5 16 ± 20 7 ± 3 6 ± 5 10 ± 1 52 ± 28 32 ± 6
  (Ct ± SD) (36.61 ± 1.44) (37.87 ± 1.13) (36.65 ± 0.6) (37.19 ± 1.21) (36.06 ± 0.2) (33.89 ± 0.98) (34.41 ± 0.2)
  DY (%) 4.48 6.81 7.82 13.78 20.11 36.31 34.48
High copy copy num. ± SD 11 503 ± 6 190 17479 ± 1 861 20 053 ± 1 168 35 342 ± 4 588 51 592 ± 2 574 93 150 ± 10 206 88 458 ± 6 161
  (Ct ± SD) (26.28 ± 0.99) (25.51 ± 0.11) (25.21 ± 0.09) (24.37 ± 0.2) (23.8 ± 0.07) (22.93 ± 0.16) (23 ± 0.01)
GNPDA copy num. ± SD 0 ± 0 26 ± 3 99 ± 12 142 ± 24 229 ± 31 1 653 ± N/A 5 314 ± 1 090
  (Ct ± SD) (40 ± 0) (33.27 ± 0.29) (30.98 ± 0.16) (30.35 ± 0.29) (29.55 ± 0.22) (26.22 ± N/A) (24.29 ± 0.37)
PowerScript         
  DY (%) 1.21 3.97 5.12 3.61 3.61 17.67 8.23
Low copy copy num. ± SD 3 ± 1 10 ± 5 13 ± 3 9 ± 3 11 ± 5 45 ± 18 21 ± 5
  (Ct ± SD) (38.34 ± 0.47) (36.48 ± 0.9) (35.99 ± 0.41) (36.56 ± 0.58) (36.35 ± 0.76) (34.17 ± 0.75) (35.27 ± 0.37)
  DY (%) 9.29 12.89 23.99 18.69 27.28 54.55 59.43
High copy copy num. ± SD 23 837 ± 723 33 078 ± 4 291 61 560 ± 340320 47 944 ± 9 523 69 982 ± 20 743 139 950 ± 10 479 152 480 ± 9 523
  (Ct ± SD) (6.01 ± 0.05) (25.53 ± 0.2) (24.72 ± 0.77) (23.2 ± 0.09) (24.42 ± 0.45) (23.33 ± 0.12) (23.2 ± 0.09)
GNPDA copy num. ± SD 2 ± 1 31 ± 3 168 ± 16 35 ± 8 58 ± 40 2 440 ± 650 6 118 ± 308
  (Ct ± SD) (36.85 ± 1.26) (32.47 ± 0.18) (30 ± 0.11) (32.29 ± 0.35) (31.9 ± 1.39) (26.07 ± 0.38) (24.69 ± 0.07)
  1. aRT systems tested were Sensiscript. Omniscript. SuperScript II. SuperScript III. and PowerScript; low copy. RT samples spiked with 2.6 × 103 EGFP mRNA copies; high copy. RT samples spiked with 2.6 × 106 EGFP mRNA copies.
  2. bDY (detection yield) was calculated using the equation described in Results and the copy number (num.) was derived from the calibration curve for a given cycle threshold value (CT) [see Additional file 2];SD. standard deviation.