Background RNA (ng) | 0 | 10 | 25 | 50 | 100 | 1000 | 2000 | |
---|---|---|---|---|---|---|---|---|
RT systemsa | Datab | |||||||
SuperScript III | ||||||||
DY (%) | 3.75 | 6.30 | 2.90 | 2.49 | 4.07 | 20.37 | 12.37 | |
Low copy | copy num. ± SD | 10 ± 5 | 16 ± 20 | 7 ± 3 | 6 ± 5 | 10 ± 1 | 52 ± 28 | 32 ± 6 |
(Ct ± SD) | (36.61 ± 1.44) | (37.87 ± 1.13) | (36.65 ± 0.6) | (37.19 ± 1.21) | (36.06 ± 0.2) | (33.89 ± 0.98) | (34.41 ± 0.2) | |
DY (%) | 4.48 | 6.81 | 7.82 | 13.78 | 20.11 | 36.31 | 34.48 | |
High copy | copy num. ± SD | 11 503 ± 6 190 | 17479 ± 1 861 | 20 053 ± 1 168 | 35 342 ± 4 588 | 51 592 ± 2 574 | 93 150 ± 10 206 | 88 458 ± 6 161 |
(Ct ± SD) | (26.28 ± 0.99) | (25.51 ± 0.11) | (25.21 ± 0.09) | (24.37 ± 0.2) | (23.8 ± 0.07) | (22.93 ± 0.16) | (23 ± 0.01) | |
GNPDA | copy num. ± SD | 0 ± 0 | 26 ± 3 | 99 ± 12 | 142 ± 24 | 229 ± 31 | 1 653 ± N/A | 5 314 ± 1 090 |
(Ct ± SD) | (40 ± 0) | (33.27 ± 0.29) | (30.98 ± 0.16) | (30.35 ± 0.29) | (29.55 ± 0.22) | (26.22 ± N/A) | (24.29 ± 0.37) | |
PowerScript | ||||||||
DY (%) | 1.21 | 3.97 | 5.12 | 3.61 | 3.61 | 17.67 | 8.23 | |
Low copy | copy num. ± SD | 3 ± 1 | 10 ± 5 | 13 ± 3 | 9 ± 3 | 11 ± 5 | 45 ± 18 | 21 ± 5 |
(Ct ± SD) | (38.34 ± 0.47) | (36.48 ± 0.9) | (35.99 ± 0.41) | (36.56 ± 0.58) | (36.35 ± 0.76) | (34.17 ± 0.75) | (35.27 ± 0.37) | |
DY (%) | 9.29 | 12.89 | 23.99 | 18.69 | 27.28 | 54.55 | 59.43 | |
High copy | copy num. ± SD | 23 837 ± 723 | 33 078 ± 4 291 | 61 560 ± 340320 | 47 944 ± 9 523 | 69 982 ± 20 743 | 139 950 ± 10 479 | 152 480 ± 9 523 |
(Ct ± SD) | (6.01 ± 0.05) | (25.53 ± 0.2) | (24.72 ± 0.77) | (23.2 ± 0.09) | (24.42 ± 0.45) | (23.33 ± 0.12) | (23.2 ± 0.09) | |
GNPDA | copy num. ± SD | 2 ± 1 | 31 ± 3 | 168 ± 16 | 35 ± 8 | 58 ± 40 | 2 440 ± 650 | 6 118 ± 308 |
(Ct ± SD) | (36.85 ± 1.26) | (32.47 ± 0.18) | (30 ± 0.11) | (32.29 ± 0.35) | (31.9 ± 1.39) | (26.07 ± 0.38) | (24.69 ± 0.07) |