Table 1 Primer sequences for MS-PCR, PSQ, BS and MSRE-PCR
Gene | Primer Sequence (5' – 3') | Technique | Methylated (m)/Unmethylated (u) | Reference | Base pair location | |
|---|---|---|---|---|---|---|
BRCA-1 | F | GGTTAATTTAGAGTTTCGAGAGACG | MS-PCR | m | Genbank: NT_010755.15 | 5001854 – 5001830 |
|  | R | TCAACGAACTCACGCCGCGCAATCG |  | m |  | 5001697 – 5001673 |
|  | F | GGTTAATTTAGAGTTTTGAGAGATG |  | u |  | 5001854 – 5001830 |
|  | R | TCAACAAACTCACACCACACAATCA |  | u |  | 5001697 – 5001673 |
MMP-2 | F | GGACGTTAAGGGTTTAGAGC | MS-PCR | m | Genbank: NT_010498.15 | 9127002 – 9127021 |
|  | R | CAATACACGACCTCGTCAC |  | m |  | 9127086 – 9127104 |
|  | F | GGATGTTAAGGGTTTAGAGT |  | u |  | 9127002 – 9127021 |
|  | R | CAATACACAACCTCATCAC |  | u |  | 9127086 – 9127104 |
BRCA-1-PSQ- PCRa | F | TAGGGGGTAGATTGGGTGGTTA | PSQ |  | Genbank: NT_010755.15 | 5001871 – 5001850 |
|  | R | CCCCCTCCAAAAAATCTCA |  |  |  | 5001675 – 5001656 |
BRCA-1-PSQ-Sa |  | TGGGTGGTTAATTTAGAGT |  |  |  | 5001859 – 5001841 |
BRCA-1-PSQ-PCRb | F | TGAGAGTAGGGGTTTAGTTATTTGAGAA | PSQ |  |  | 5001614 – 5001641 |
|  | R | TTTCTATCCCTCCCATCCTCTAATTAT |  |  |  | 5001795 – 5001821 |
BRCA-1-PSQ-Sb |  | TTTGTTTTTAGTTTAGGAAG |  |  |  | 5001651 – 5001670 |
MMP-14 | F | TTGTAATTGGATTTAGGTTAAAA | BS |  | Genbank: NT_026437.11 | 4305511 – 4305533 |
|  | R | AACACTAAACTTAAATTCCTAAACC |  |  |  | 4305741 – 4305765 |
MMP-1 | F | CCAGGCCTCAGTGGAGCTA | MSRE-PCR |  | Genbank: NT_033899.7 | 6233232 – 6233214 |
|  | R | AATGGGAAGACATTCTCACGA |  |  |  | 6233000 – 6232982 |
MMP-3 | F | CAACTTCAAAGCATCTGCTAATT | MSRE-PCR |  | Genbank: NT_033899.7 | 6277588 – 6277566 |
|  | R | ATGGGCAGAATAGAACAAAGAGG |  |  |  | 6277355 – 6277333 |