Figure 4

C/EBPδ is bound to and regulates the ΔNp63 promoter. A. Left Panel. Human KCs were transfected with mouse C/EBPδ. RNAs were extracted at the indicated time and RT-PCR is shown for the indicated genes: controls (mC/EBPδ, GAPDH) and different p63 isoform (upper Panels). Right Panel. RNAi of C/EBPδ with three different oligos. C/EBPδ mRNA was checked for inhibition. B. Bioinformatic analsysis of the ΔNp63 promoters for C/EBP sites with different algorithms: a cluster of sites commonly detected at position -2400 is shown. Analysis was performed with rVista [63], CONSITE [64] and CUSTOM. Shown are also mouse conservation and a schematic representation of the amplified region. C. Transfection analysis of ΔNp63 constructs [22] without and with NF-Y and/or C/EBPδ. D. ChIP analysis of the upstream regions of ΔNp63 with the indicated antibodies, using chromatin from primary KCs.