Figure 5
From: GATA elements control repression of cardiac troponin I promoter activity in skeletal muscle cells
GATA-3 and -4 proteins are not detected and GATA-2 protein is barely detected by Western blotting in skeletal muscle cells. Western blotting was performed using antibodies specific for GATA-2, -3 or 4 and nuclear extracts of differentiated C2C12 cells (M) or control cells (He:Hela, J:Jurkat or H:heart cells). A control blot reacted with anti-MEF2 antibody demonstrates the presence of MEF2 in nuclear extracts of muscle cells. 20 μg of extract was loaded per lane.