Muscle cells express GATA-2, -3 and -4 but not GATA-1, -5 and -6 transcripts. A. RT-PCR assays were performed with RNA preparations from differentiated C2C12 cells (M), using heart (H), liver (L) or brain (B) RNA preparations as positive controls. The primers used are indicated in the Method section. Note that muscle cells express GATA-2, -3 and -4 but not GATA-1, -5 and -6 transcripts. B. Relative mRNA expression levels of GATA transcripts were evaluated by quantitative real-time PCR, using cyclophilin A as an internal standard.