Figure 4

Validation of the RdCVF2 expression in retina. Panel a shows the expression of the short and long forms of RdCVF2 mRNA in the wild-type and rd1 retina at post-natal day 35. The RT-PCR product lengths are (RdCVF2-S: 176 bp, RdCVF2-L: 170 bp. Panel b: northern blotting analysis of RdCVF2 'exon 1) and G6PDH control. Panel c: In situ hybridization on sections of wild-type and rd1 mice retina with digoxigenin-labeled RdCVF2-S and L riboprobes (AS : antisens, S : sens). Panel d shows expression time-courses for both isoforms of RdCVF2 and rhodopsin in wild-type and rd1 mice during the first postnatal month. GC, Ganglion cells; INL, inner nuclear layer; ONL, outer nuclear layer. Original magnification : 40 ×.