Table 2 List of primers used for reverse transcriptase-polymer chain reactions, site-directed deletion mutagenesis, and ribonuclease protection assay

RT-PCR

TβRI

S:

5'-CGAGGTTTGCTGGGGTGAGGCA-3'

AS:

5'-CTCAGTGAGGTAGAACAACTGACC-3'

S:

5'-AACAAGCTTTCCACCATGGAGGCGGCGGT-3'

AS:

5'-GTTCATATCTTACATTTTGATGCCTTCCTGT-3'

β-Actin

S:

5'-CCCTTTTTGTCCCCCAAC-3'

AS:

5'-CTGGTCTCAAGTCAGTGTACAGGT-3'

TβRI-HA

S:

5'-ATCTATGCAATGGGCTT-3'

AS:

5'-AGCGTAATCTGGAACATCGTA-3'

Mutagenesis

TβRI

S:

5'-ATGGAGGCGGCGGTCGCGGCGGCGGCGGCGGCG-3'

AS:

5'-CGCCGCCGCCGCCGCCGCGACCGCCGCCTCCAT-3'

S:

5'-CTGCTCCTCCTCGTGCTGCTGCTCCCGGGGGCGACG-3'

AS:

5'-CGTCGCCCCCGGGAGCAGCAGCACGAGGAGGAGCAG-3'

S:

5'-GCGGCGGCGGCGGCGGCGCTCTGTACAAAAGACAAT-3'

AS:

5'-ATTGTCTTTTGTACAGAGCGCCGCCGCCGCCGCCGC-3'

RPA

TβRI

S:

5'-ACCATGGAGGCGGCGGT-3'

AS:

5'-TAATACGACTCACTATAGGGACCACCACCTCCTTATTGC-AATGGTCCTGA-3'

S:

5'-ACCATGGAGGCGGCGGT-3'

AS:

5'-TAATACGACTCACTATAGGGACCACCACCTCCAGAGGGT-GCACATACAA-3'

S:

5'-AACAAGCTTTCCACCATGGAGGCGGCGGTCGC-3'

AS:

5'-TAATACGACTCACTATAGGGACCACCACCTCCTTCAGCT-ATACACATGCT-3'

S:

5'-AACAAGCTTTCCACCATGGAGGCGGCGGTCGC-3'

AS:

5'-TAATACGACTCACTATAGGGACCACCACCTCCATCTCTA-TGAGCAATGGCT-3'