Table 5 Oligonucleotide primers used for RACE and PCR in this study
Name | Target | Sequence (5'-3') |
|---|---|---|
Forward primers | Â | Â |
   Ex1:1Fw | exon 1 | 5'-TTAAGCTTGGTACCCGCGGACCTGGGGATTAATGG |
   Ex1:2 Fw | exon 1 | 5'-AATTCTGCGGAGCCTGCGG |
   Ex2Fw | exon 2 | 5'-ACGTGATACTCACACAGTGG |
   Ex2a:1Fw | exon 2a | 5'-GGAGCTCCAGGGAATACAATGTTCA |
   Ex2a:2Fw | exon 2a | 5'-CAGGCCCAGTGGCAGCAAT |
   Ex2cFw | exon 2c | 5'-GGCACTTGATGCTGTTGAATGGAG |
   Ex2eFw | exon 2e | 5'-CCAGCTCTCCTTGACCTTGAACCTT |
   Ex3Fw | exon 3 | 5'-AACTGAAGCCCGTGGAGCA |
   Ex4Fw | exon 4 | 5'-GACCACAGCATGCACTTCCT |
   Ex8:1Fw | exon 8 | 5'-AGTGGCTTTGTCACCCGTGAGTTCCTG |
   Ex8:2Fw | exon 8 | 5'-TCCGCAAACCCTTCAGTGATA |
   In8Fw | intron 8 * | 5'-GGCTGAGCCAGGAGAATCGCTTGA |
Reverse primers | Â | Â |
   Ex2Rev | exon 2 | 5'-CTCGTTGGTGCATCTGTCTTCA |
   Ex2a:1Rev | exon 2a | 5'-CGCTCAGGAGGTACTTTGACCTCTTCC |
   Ex2a:2Rev | exon 2a | 5'-GCTCAGGGATGAACTGGACAACTGAC |
   Ex2cRev | exon 2c | 5'-ATGCTTCTGCTCAGAACCCAGCTCTCA |
   Ex2eRev | exon 2e | 5'-AAGAGTGAAGGGCAGCTGAGTCCTGA |
   Ex3Rev | exon 3 | 5'-GAAGCAGTCCTGTAGAGATCAT |
   Ex4:1Rev | exon 4 | 5'-CCCAGTCATAGCTCTGGCATCGTCT |
   Ex4:2Rev | exon 4 | 5'-TGGCTGCTCCATGGCTGATCT |
   Ex5Rev | exon 5 | 5'-GTCACAGCCCATCTGCAGTT |
   Ex6Rev | exon 6 | 5'-CACTTCTCGTACTCCAGCTTC |
   Ex7Rev | exon 7 | 5'-TGCCACCAGCTTCCTCTTC |
   Ex8Rev | exon 8 | 5'-CCAGCCCCTTCTCTGCCT |
   Ex9:1Rev | exon 9 | 5'-CTGGAGCAGAGGGTGCAGCGAGGTCT |
   Ex9:2Rev | exon 9 | 5'-ATGGCATGCCCAGCTGTGC |
   In8:1Rev | intron 8* | 5'-GATTACAGGTGTGAGCCACCACGTCTG |
   In8:2Rev | intron 8* | 5'-CCCACATTAACATGACAGCAT |