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Table 5 Oligonucleotide primers used for RACE and PCR in this study

From: Alternative splicing of human peroxisome proliferator-activated receptor delta (PPARdelta):effects on translation efficiency and trans-activation ability

Name Target Sequence (5'-3')
Forward primers   
   Ex1:1Fw exon 1 5'-TTAAGCTTGGTACCCGCGGACCTGGGGATTAATGG
   Ex1:2 Fw exon 1 5'-AATTCTGCGGAGCCTGCGG
   Ex2Fw exon 2 5'-ACGTGATACTCACACAGTGG
   Ex2a:1Fw exon 2a 5'-GGAGCTCCAGGGAATACAATGTTCA
   Ex2a:2Fw exon 2a 5'-CAGGCCCAGTGGCAGCAAT
   Ex2cFw exon 2c 5'-GGCACTTGATGCTGTTGAATGGAG
   Ex2eFw exon 2e 5'-CCAGCTCTCCTTGACCTTGAACCTT
   Ex3Fw exon 3 5'-AACTGAAGCCCGTGGAGCA
   Ex4Fw exon 4 5'-GACCACAGCATGCACTTCCT
   Ex8:1Fw exon 8 5'-AGTGGCTTTGTCACCCGTGAGTTCCTG
   Ex8:2Fw exon 8 5'-TCCGCAAACCCTTCAGTGATA
   In8Fw intron 8 * 5'-GGCTGAGCCAGGAGAATCGCTTGA
Reverse primers   
   Ex2Rev exon 2 5'-CTCGTTGGTGCATCTGTCTTCA
   Ex2a:1Rev exon 2a 5'-CGCTCAGGAGGTACTTTGACCTCTTCC
   Ex2a:2Rev exon 2a 5'-GCTCAGGGATGAACTGGACAACTGAC
   Ex2cRev exon 2c 5'-ATGCTTCTGCTCAGAACCCAGCTCTCA
   Ex2eRev exon 2e 5'-AAGAGTGAAGGGCAGCTGAGTCCTGA
   Ex3Rev exon 3 5'-GAAGCAGTCCTGTAGAGATCAT
   Ex4:1Rev exon 4 5'-CCCAGTCATAGCTCTGGCATCGTCT
   Ex4:2Rev exon 4 5'-TGGCTGCTCCATGGCTGATCT
   Ex5Rev exon 5 5'-GTCACAGCCCATCTGCAGTT
   Ex6Rev exon 6 5'-CACTTCTCGTACTCCAGCTTC
   Ex7Rev exon 7 5'-TGCCACCAGCTTCCTCTTC
   Ex8Rev exon 8 5'-CCAGCCCCTTCTCTGCCT
   Ex9:1Rev exon 9 5'-CTGGAGCAGAGGGTGCAGCGAGGTCT
   Ex9:2Rev exon 9 5'-ATGGCATGCCCAGCTGTGC
   In8:1Rev intron 8* 5'-GATTACAGGTGTGAGCCACCACGTCTG
   In8:2Rev intron 8* 5'-CCCACATTAACATGACAGCAT
  1. The primers are annotated according to the target exon or intron in the human PPARδ gene.
  2. * Comparable to the 3'-UTR of PPARδ2